Isolation, propagation, and titration of human immunodeficiency virus type 1 from peripheral blood of infected individuals.
نویسندگان
چکیده
HIV-1 can be isolated from peripheral blood mononuclear cells and is easily propagated on primary cells in vitro. Here we describe the method for bulk isolation of the HIV-1 quasispecies and a limiting dilution virus isolation protocol by which single coexisting clones can be obtained. In addition, methods for propagation and titration of HIV-1 are provided.
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ورودعنوان ژورنال:
- Methods in molecular biology
دوره 304 شماره
صفحات -
تاریخ انتشار 2005